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Human SOST/Sclerostin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1406

R&D Systems, part of Bio-Techne
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MAB1406-100
MAB1406-500
MAB1406-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Western Blot

Cited:

ELISA Development, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 220902

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SOST/Sclerostin
Gln24-Tyr213
Accession # Q9BQB4

Specificity

Detects human SOST/Sclerostin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SOST is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human SOST/Sclerostin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human SOST/Sclerostin (Catalog # 1406-ST)

Human SOST Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human SOST/Sclerostin Biotinylated Antibody (Catalog # BAM14061)
  • Standard: Recombinant Human SOST/Sclerostin Protein (Catalog # 1406-ST)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

SOST, also known as sclerostin, belongs to the Cerberus/DAN family of secreted glycoproteins. SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity and functions in bone homeostasis as a BMP antagonist.

Alternate Names

sclerostin, VBCHsclerosteosis

Entrez Gene IDs

50964 (Human); 74499 (Mouse)

Gene Symbol

SOST

UniProt

Additional SOST/Sclerostin Products

Product Documents for Human SOST/Sclerostin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SOST/Sclerostin Antibody

For research use only

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