Human SOST/Sclerostin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM14061

R&D Systems, part of Bio-Techne
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BAM14061
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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 220910

View all SOST/Sclerostin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SOST/Sclerostin
Gln24-Tyr213
Accession # Q9BQB4

Specificity

Detects human SOST/Sclerostin in ELISAs. In sandwich immunoassays, 30% cross-reactivity with recombinant mouse SOST is observed and no cross-reactivity or interference with recombinant human BMP-2, -4, -5, -6, or -7 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human SOST/Sclerostin Biotinylated Antibody

Application
Recommended Usage

Human SOST Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human SOST/Sclerostin Antibody (Catalog # MAB1406)
  • Standard: Recombinant Human SOST/Sclerostin Protein (Catalog # 1406-ST)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1‑3).

References

  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

Alternate Names

sclerostin, VBCHsclerosteosis

Entrez Gene IDs

50964 (Human); 74499 (Mouse)

Gene Symbol

SOST

UniProt

Q9BQB4

Additional SOST/Sclerostin Products

Product Documents for Human SOST/Sclerostin Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SOST/Sclerostin Biotinylated Antibody

For research use only

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