Human SOX17 APC-conjugated Antibody

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry.

Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human SOX17 by Flow Cytometry

Addition of DMSO to Activin A/low serum-based medium increases the efficiency of definitive endoderm differentiation.(A) Flow cytometry analysis of hESC and DE cells double-stained for the pluripotency marker OCT4 and the endoderm marker SOX17. Pluripotency and differentiation status in all culture conditions tested are shown as contour plots. Only upon addition of DMSO (0.5%, 1%) to Activin A-driven differentiation of hESC was the number of OCT4-positive cells significantly down regulated. Culture with 1% of DMSO yielded the highest number of SOX17+, OCT4- cells. ‘Null’ represents spontaneous differentiation with no differentiating growth factors/reagents added. Each contour plot represents 3 biological replicates pooled together. (B) Immunofluorescence staining of cells at day 2 of DE formation for OCT4 and SOX17 markers with DAPI nuclear staining. Cells cultured in Activin A alone were clearly separated into two widespread populations of SOX17- or OCT4-positive cells (B-A). DMSO in the absence of Activin A does not prime cells to acquire endoderm identity (B-B). Addition of 0.5% of DMSO to Activin A-based, DE-priming medium results in significant decrease of OCT4-positive clusters of cells as early as 48h after DE differentiation was initiated (B-C). Scale bar 100μm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117689), licensed under a CC-BY license. Not internally tested by R&D Systems.