Detects human SOX9 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) SOX10 is observed and less than 5% cross-reactivity with rhSOX2, rhSOX3, rhSOX7, and rhSOX17 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SOX9 NorthernLights™ NL557-conjugated Antibody

SOX9 in U-251 MG Human Cell Line.

SOX9 was detected in immersion fixed U-251 MG human glioblastoma cell line using Goat Anti-Human SOX9 NorthernLights™ NL557-conjugated Antigen Affinity-purified Polyclonal Antibody (red, Catalog # NL3075R) at 5 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human SOX9 NorthernLights™ NL557-conjugated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U-251 MG human glioblastoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Entrez Gene IDs

6662 (Human)

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for Human SOX9 NorthernLights™ NL557-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SOX9 NorthernLights™ NL557-conjugated Antibody

For research use only

Citations
Reviews

Human SOX9 NorthernLights™ NL557-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # NL3075R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SOX9 Antibodies »

Product Specifications

Immunogen

Specificity