Human STAT2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB16662

R&D Systems, part of Bio-Techne
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MAB16662-100
MAB16662-SP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 967425

View all STAT2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT2
Gln679-Phe851
Accession # P52630

Specificity

Detects human STAT2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human STAT2 Antibody

Detection of Human STAT2 antibody by Western Blot.

Detection of Human STAT2 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human STAT2 antibody by Simple WesternTM.

Detection of Human STAT2 by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT2 at approximately 110 kDa (as indicated) using 10 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Show Human STAT2 Antibody Specificity Using Knockout Cell Line.

Western Blot Show Human STAT2 Specificity Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human STAT2 Antibody

Application
Recommended Usage

Knockout Validated

STAT2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT2 knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT2

Signal transducer and activator of transcription 2 (STAT2) is a member of the transcription factor STAT family with an apparent M.W. of 113 kDa in SDS-PAGE gels. Human STAT2 is 851 amino acids (aa) in length and contains one SH2 domain (aa 572-667). Splicing variants produce two isoforms for human STAT2. The short isoform has a 32 aa substitution and a 199 aa deletion corresponding to aa 621‑652 and aa 653‑851 in the long isoform, respectively. Human STAT2 shares 73% and 65% aa sequence identity with rat and mouse STAT2, in that order. STAT2 functions as a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta), and it is also required for myogenic differentiation.

Long Name

Signal Transducer and Activator of Transcription 2

Alternate Names

interferon alpha induced transcriptional activator, ISGF-3, MGC59816, p113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113

Entrez Gene IDs

6773 (Human); 20847 (Mouse)

Gene Symbol

STAT2

UniProt

P52630

Additional STAT2 Products

Product Documents for Human STAT2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STAT2 Antibody

For research use only

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