Human Survivin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8861
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1277A
Product Specifications
Immunogen
Full length recombinant human Survivin
Accession # O15392
Accession # O15392
Specificity
Detects human Survivin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Survivin Antibody
Detection of Human Survivin by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 786-O human renal cell adenocarcinoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Survivin Polyclonal Antibody (Catalog # MAB8861) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Survivin at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Survivin in MCF‑7 Human Cell Line.
Survivin was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human Survivin Polyclonal Antibody (Catalog # MAB8861) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Survivin in Human Breast Cancer Tissue.
Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Survivin Monoclonal Antibody (Catalog # MAB8861) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Survivin Antibody
Application
Recommended Usage
Immunocytochemistry
1 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 786‑O human renal cell adenocarcinoma cell line, and Bowes human melanoma cell line
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 786‑O human renal cell adenocarcinoma cell line, and Bowes human melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB8861 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Survivin
Additional Survivin Products
Product Documents for Human Survivin Antibody
Product Specific Notices for Human Survivin Antibody
For research use only
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