Human Syntaxin-BP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5659
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Syntaxin‑BP3
Thr84-Thr231
Accession # O00186
Thr84-Thr231
Accession # O00186
Specificity
Detects human Syntaxin‑BP3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) STXBP1 and rhSTXBP2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Syntaxin-BP3 Antibody
Detection of Human Syntaxin-BP3 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntaxin-BP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5659) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Syntaxin-BP3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin-BP3
Syntaxin‑BP3 (Syntaxin-binding protein 3; also PSP, Munc18-C and -3; and Unc-18C) is a 66‑70 kDa member of the SNARE-interacting Sec1/Munc18 family of proteins. It is expressed in cells that exhibit granule exocytosis, such as neutrophils, mast cells, platelets and endothelial cells. Functionally, it likely participates in the consolidation and secretion of secondary and tertiary granules. Human Syntaxin‑BP3 is 592 amino acids (aa) in length. It contains one SEC1 domain (aa 16‑592). Phosphorylation at Ser129 may stimulate granule release. There are two potential splice variants. One shows a 12 aa substitution for aa 31‑269, while a second shows a one aa Gln insertion after Lys270. Over aa 84‑231, human Syntaxin‑BP3 shares 90% aa identity with mouse Syntaxin‑BP3.
Long Name
Syntaxin-binding Protein 3
Alternate Names
Munc18c, STXBP3, SyntaxinBP3, Unc-18C
Gene Symbol
STXBP3
UniProt
Additional Syntaxin-BP3 Products
Product Documents for Human Syntaxin-BP3 Antibody
Product Specific Notices for Human Syntaxin-BP3 Antibody
For research use only
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