Detects human TACI/TNFRSF13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BCMA or recombinant mouse TACI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TACI/TNFRSF13B Antibody

Detection of TACI/TNFRSF13B in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TACI/TNFRSF13B Monoclonal Antibody (Catalog # MAB1741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114).

Applications for Human TACI/TNFRSF13B Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Raji human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB1741 in the following applications:

Functional Assay (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science, 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Alternate Names

CD267, CVID, TNFRSF13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Gene Symbol

TNFRSF13B

UniProt

O14836

Additional TACI/TNFRSF13B Products

Product Documents for Human TACI/TNFRSF13B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TACI/TNFRSF13B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TACI/TNFRSF13B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1741

Key Product Details

Species Reactivity

Validated:

Cited:

Applications