Human TAK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5307
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 491840
Product Specifications
Immunogen
E. coli-derived recombinant human TAK1 isoform 1A
Met1-Ser579
Accession # Q5U0D0
Met1-Ser579
Accession # Q5U0D0
Specificity
Detects endogenous human TAK1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TAK1 Antibody
Detection of Human TAK1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human TAK1 Monoclonal Antibody (Catalog # MAB5307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAK1 at approximately 80 to 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TAK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TAK1
TGF-beta-activated kinase 1 (TAK1; also MAP3K7) is a member of the STE Ser/Thr protein kinase family and the MAP kinase kinase kinase subfamily. Human TAK1 is 606 amino acids (aa) in length and contains a protein kinase domain at aa 36‑291. There are three isoforms for TAK1. Isoform 1B is the largest isoform. Isoform 1A has a 27 aa deletion corresponding to aa 404‑430 in isoform 1B, and isoform 1C has an 88 aa deletion corresponding to aa 519‑606 in isoform 1B. TAK1 activity has been implicated during TGF beta-induced and Ras-dependent tumorigenesis.
Long Name
TGF-beta activated kinase 1
Alternate Names
MAP3K7, TGF1a
Gene Symbol
MAP3K7
UniProt
Additional TAK1 Products
Product Documents for Human TAK1 Antibody
Product Specific Notices for Human TAK1 Antibody
For research use only
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