Human TCF7L1/TCF3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6116

R&D Systems, part of Bio-Techne
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MAB6116
MAB6116-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 622722

View all TCF7L1/TCF3 Antibodies »

Product Summary for Human TCF7L1/TCF3 Antibody

Immunogen

E. coli-derived recombinant human TCF7L1/TCF3
Lys429-Ser581
Accession # Q9HCS4

Specificity

Detects human TCF7L1/TCF3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TCF7L1/TCF3 Antibody

Detection of Human TCF7L1 antibody by Western Blot.

Detection of Human TCF7L1 by Western Blot.

Western blot shows lysates of human lung tissue, human pancreas tissue, and human spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TCF7L1/TCF3 Monoclonal Antibody (Catalog # MAB6116) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF7L1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TCF7L1/TCF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human lung tissue, human pancreas tissue, and human spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins. Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

Long Name

Transcription Factor 7-like 1

Alternate Names

TCF3

Entrez Gene IDs

83439 (Human); 21415 (Mouse); 312451 (Rat)

Gene Symbol

TCF7L1

UniProt

Q9HCS4 (Human)

Additional TCF7L1/TCF3 Products

Product Documents for Human TCF7L1/TCF3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TCF7L1/TCF3 Antibody

For research use only

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