Human TEM8/ANTXR1 Isoform 1 Antibody

Name: Human TEM8/ANTXR1 Isoform 1 Antibody
Brand: R&D Systems
SKU: AF3886

R&D Systems, part of Bio-Techne | Catalog # AF3886

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TEM8/ANTXR1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TEM8/ANTXR1
Gln28-Gly320
Accession # Q9H6X2

Specificity

Detects human TEM8/ANTXR1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TEM8/ANTXR1 Isoform 1 Antibody

Detection of Human TEM8 Isoform 1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TEM8 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3886) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TEM8 at approximately 55 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

TEM8 in HT-29 Human Cell Line.

TEM8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Goat Anti-Human TEM8 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3886) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TEM8/ANTXR1 in Human Liver Cancer Tissue.

TEM8/ANTXR1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human TEM8/ANTXR1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3886) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TEM8/ANTXR1 Isoform 1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue and human liver cancer tissue

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM8/ANTXR1

TEM8, also called ATR1, is a glycoprotein of the anthrax toxin receptor family that is expressed by endothelial cells. Type I transmembrane isoforms of 564 aa (80‑85 kDa) and 368 aa (60 kDa) and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) are differentially expressed. All diverge at the C-terminal end but share the N-terminal vWFA domain that binds the anthrax toxin PA subunit as well as collagens I and IV. Human TEM8 shares 99% aa identity with mouse and rat and 92% identity with canine and chick TEM8 within the extracellular domain.

Long Name

Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

Alternate Names

ANTXR1

Entrez Gene IDs

84168 (Human); 69538 (Mouse); 362393 (Rat)

Gene Symbol