Human Thrombopoietin/Tpo Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB288
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 34817
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo
Ser22-Gly353
Accession # P40225
Ser22-Gly353
Accession # P40225
Specificity
Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Thrombopoietin/Tpo Antibody
Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody.
Recombinant Human Thrombo-poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND50 is typically 5-10 µg/mL.Applications for Human Thrombopoietin/Tpo Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.
Human Thrombopoietin/Tpo Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombopoietin/Tpo
Alternate Names
MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo
Gene Symbol
THPO
UniProt
Additional Thrombopoietin/Tpo Products
Product Documents for Human Thrombopoietin/Tpo Antibody
Product Specific Notices for Human Thrombopoietin/Tpo Antibody
For research use only
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