Human TIMP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB970
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate
Applications
Validated:
CyTOF-ready, ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Cell-based ELISA, Dot Blot, ELISA Capture, Immunocytochemistry, Luminex Development, Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 63515
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # P01033
Cys24-Ala207
Accession # P01033
Specificity
Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse TIMP‑1 or recombinant human TIMP‑2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TIMP-1 Antibody
TIMP‑1 in Wi-38 Human Cell Line.
TIMP-1 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TIMP-1 in Human Astrocytoma.
TIMP-1 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human TIMP-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed Wi-38 human lung fibroblast cell line
Sample: Immersion fixed Wi-38 human lung fibroblast cell line
Immunohistochemistry
8-25 µg/mL
Sample:
Sample:
Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and astrocytoma tissue
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human T cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human T cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human TIMP-1 Western Blot Standard (Catalog # WBC021)
under non-reducing conditions only
Sample: Recombinant Human TIMP-1 Western Blot Standard (Catalog # WBC021)
under non-reducing conditions only
Human TIMP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB970 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Human TIMP-1 Antibody
Product Specific Notices for Human TIMP-1 Antibody
For research use only
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