Human TIMP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF970
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Knockout Validated, Neutralization, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, In vivo assay, Neutralization, Proximity Ligation Assay (PLA), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # Q6FGX5
Cys24-Ala207
Accession # Q6FGX5
Specificity
Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TIMP-1 Antibody
Detection of Human TIMP‑1 by Western Blot.
Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TIMP‑1 by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TIMP‑1 in Human Colon Cancer Tissue.
TIMP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Applications for Human TIMP-1 Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Knockout Validated
TIMP‑1
is specifically detected in SK‑OV‑3 human ovarian adenocarcinoma parental cell line but is not detectable in
TIMP‑1 knockout SK‑OV‑3 cell line.
Simple Western
50 µg/mL
Sample: PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue
Sample: PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue
Western Blot
1-5 µg/mL
Sample: Human lung tissue, human prostate tissue, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Sample: Human lung tissue, human prostate tissue, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Neutralization
Measured by its ability to neutralize Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL-Dpa-AR-NH2 (5 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using AF970 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Human TIMP-1 Antibody
Product Specific Notices for Human TIMP-1 Antibody
For research use only
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