Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human TLR7 by Flow Cytometry

Gating strategy for the detection of TLR-7 and -9 in B cell subsets.PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-7 and -9. FSC and SSC were first used to gate out debris (A) and SSC-A and SSC-H was utilized to eliminate duplicates (B). Further gating was done on CD45 and CD19, to target B cells (C). To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-7 and -9 expression on these subsets (E). Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120383), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TLR7 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Ramos human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using IC5875P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR7

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Long Name

Toll-like Receptor 7

Alternate Names

toll-like receptor 7

Entrez Gene IDs

51284 (Human); 170743 (Mouse); 317468 (Rat)

Gene Symbol

TLR7

UniProt

Q9NYK1

Additional TLR7 Products

Product Documents for Human TLR7 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TLR7 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human TLR7 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC5875P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TLR7 PE-conjugated Antibody

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry.