Human TLR9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB36582
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Accession # Q9NR96
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TLR9 Antibody
Detection of TLR9 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and (A) Mouse Anti-Human/Mouse TLR9 Monoclonal Antibody (Catalog # MAB36582) or (B) Mouse IgG1 control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human TLR9 Antibody
CyTOF-ready
Intracellular Staining by Flow Cytometry
Sample: Human PBMCs fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Reviewed Applications
Read 1 review rated 4 using MAB36582 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR9
TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123+ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that contain either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER and translocates to either the cell membrane or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818) plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16, a second possesses an alternate start site at Met58, while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189, human TLR9 shares 76% aa identity with mouse TLR9.
Long Name
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UniProt
Additional TLR9 Products
Product Documents for Human TLR9 Antibody
Product Specific Notices for Human TLR9 Antibody
For research use only