Human TRA-1-85/CD147 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM3195
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Product Specifications
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑85.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TRA-1-85/CD147 Biotinylated Antibody
TRA-1-85/CD147 in THP-1 Human Cell Line.
TRA-1-85/CD147 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human TRA-1-85/CD147 Biotinylated Monoclonal Antibody (Catalog # BAM3195) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human TRA-1-85/CD147 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells
Sample: Human peripheral blood mononuclear cells
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Western Blot
Williams, B.P. et al. (1988) Immunogenetics. 27:322. This application was not tested by R&D Systems.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRA-1-85/CD147
References
- Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Alternate Names
OKa Blood Group Antigen, TRA185
Entrez Gene IDs
682 (Human)
Gene Symbol
BSG
Additional TRA-1-85/CD147 Products
Product Documents for Human TRA-1-85/CD147 Biotinylated Antibody
Product Specific Notices for Human TRA-1-85/CD147 Biotinylated Antibody
For research use only
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