Human TRACP/PAP/ACP5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3948
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRACP/PAP/ACP5
Ala22-Pro320
Accession # P13686
Ala22-Pro320
Accession # P13686
Specificity
Detects human TRACP/PAP/ACP5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human TRACP/PAP/ACP5 Antibody
Detection of Human TRACP/PAP/ACP5 by Western Blot.
Western blot shows lysates of human lung tissue, SK-Mel-28 human malignant melanoma cell line and Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TRACP/PAP/ACP5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRACP/PAP/ACP5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TRACP/PAP/ACP5 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP), see our available Western blot detection antibodies
Western Blot
0.5 µg/mL
Sample: Human lung tissue, SK‑Mel‑28 human malignant melanoma cell line and Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP).
Sample: Human lung tissue, SK‑Mel‑28 human malignant melanoma cell line and Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRACP/PAP/ACP5
Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5) (1). The deuced amino acid (aa) sequence of human ACP5 predicts a signal peptide (aa 1 to 21) and a mature chain (aa 22 to 325). R&D Systems' recombinant human ACP5 consists of aa 22 to 320, without the last 5 residues, RRARP.
ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells (2). Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer (3).
References
- Janckila, A.J. and J.M. Halleen (2003) J. Bone Miner. Res. 18:1892.
- Halleen, J.M. et al. (2006) Clin. Lab. 52:499.
- Janckila, A.J. et al. (2003) J. Bone Miner. Res. 18:1916.
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
UniProt
Additional TRACP/PAP/ACP5 Products
Product Documents for Human TRACP/PAP/ACP5 Antibody
Product Specific Notices for Human TRACP/PAP/ACP5 Antibody
For research use only
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