Human Transglutaminase 7/TGM7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5426
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 602708
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7
Asp2-Pro710
Accession # Q96PF1
Asp2-Pro710
Accession # Q96PF1
Specificity
Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human TGM2, 3, 4, or recombinant mouse TGM2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Transglutaminase 7/TGM7 Antibody
Detection of Human Transglutaminase 7/TGM7 by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human Transglutaminase 7/TGM7 Monoclonal Antibody (Catalog # MAB5426) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Transglutaminase 7/TGM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Transglutaminase 7/TGM7 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 7/TGM7
Transglutaminase 7 (TGM7), also known as protein-glutamine gamma-glutamyltransferase Z, TGase Z and TGZ, is an 80 kDa, widely expressed member of the Transglutaminase superfamily and family. Human Transglutaminase 7 is 710 amino acids (aa) in length and contains a Transglutaminase family, C-terminal Ig like domain (aa 608‑705). Human Transglutaminase 7 shares 78% aa identity with mouse Transglutaminase 7. Like other Transglutaminases, Transglutaminase 7 is an enzyme that stabilizes protein assembly through the formation of intra- or intermolecular N epsilon( gamma-glutamyl)lysine bonds. It catalyzes a calcium-dependent transferase reaction between the gamma-carboxamide group of a peptide-bound glutamine residue and various primary amines, most commonly the epsilon-amino group of lysine residues.
Alternate Names
TGM7, TGMZ, TGZ
Gene Symbol
TGM7
UniProt
Additional Transglutaminase 7/TGM7 Products
Product Documents for Human Transglutaminase 7/TGM7 Antibody
Product Specific Notices for Human Transglutaminase 7/TGM7 Antibody
For research use only
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