Human Transthyretin/Prealbumin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7505
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Porcine
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 760430
Product Specifications
Immunogen
Human Transthyretin/Prealbumin
Specificity
Detects human Transthyretin/Prealbumin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Transthyretin/Prealbumin Antibody
Detection of Human Transthyretin/Prealbumin by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transthyretin/Prealbumin Monoclonal Antibody (Catalog # MAB7505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transthyretin/Prealbumin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Transthyretin/Prealbumin in HepG2 Human Cell Line.
Transthyretin/Prealbumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Transthyretin/ Prealbumin Monoclonal Antibody (Catalog # MAB7505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Transthyretin/Prealbumin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: Human plasma
Sample: Human plasma
Reviewed Applications
Read 1 review rated 4 using MAB7505 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transthyretin/Prealbumin
Additional Transthyretin/Prealbumin Products
Product Documents for Human Transthyretin/Prealbumin Antibody
Product Specific Notices for Human Transthyretin/Prealbumin Antibody
For research use only
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