Human Trappin‑2/Elafin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1747
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Detection, ELISA Development, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trappin-2/Elafin (R&D Systems, Catalog # 1747-PI)
Ala23-Gln117
Accession # P19957
Ala23-Gln117
Accession # P19957
Specificity
Detects human Trappin-2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM‑2, and human Neutrophil Elastase is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Trappin‑2/Elafin Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
Sample: Recombinant Human Trappin-2/Elafin (Catalog # 1747-PI)
Human Trappin-2/Elafin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BAF1747 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trappin-2/Elafin
Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72-117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally.
References
- Schalkwijk, J. et al. (1999) Biochem. J. 340:569.
Long Name
Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
Alternate Names
Elafin, ESI, PI3, SKALP, Trappin2
Entrez Gene IDs
5266 (Human)
Gene Symbol
PI3
UniProt
Additional Trappin-2/Elafin Products
Product Documents for Human Trappin‑2/Elafin Biotinylated Antibody
Product Specific Notices for Human Trappin‑2/Elafin Biotinylated Antibody
For research use only
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