Human TRIM32 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6515
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 717801
Product Summary for Human TRIM32 Antibody
Immunogen
E. coli-derived recombinant human TRIM32
Arg105-Lys204
Accession # Q13049
Arg105-Lys204
Accession # Q13049
Specificity
Detects human TRIM32 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TRIM32 Antibody
Detection of Human TRIM32 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human TRIM32 Monoclonal Antibody (Catalog # MAB6515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM32 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TRIM32 Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC‑3 human prostate cancer cell line
Sample: A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC‑3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRIM32
TRIM32 (Tripartite motif-containing protein 32; also 72 kDa TAT-interacting protein, and zinc finger protein HT2A) is a 72-82 kDa member of the TRIM/RBCC family of proteins. It is an E3 ligase that is found in structures termed nuclear and cytoplasmic bodies. Cells known to express TRIM32 are diverse and include fibroblasts, keratinocytes, skeletal muscle cells and neurons. TRIM32 ubiquitinates select proteins such as c-myc, Abi2, actin and dysbindin. Human TRIM32 is 653 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 20-65), a B-Box type zinc-finger region (aa 103-133), a coiled-coil region (aa 138-197), five NHL repeats (aa 358-646) and three utilized phosphorylation sites (Ser328/335/339). TRIM32 has the potential to form homomultimers. Over aa 105-204, human TRIM32 exhibits 95% aa identity with mouse TRIM32.
Long Name
Tripartite Motif-containing Protein 32
Alternate Names
BBS11, HT2A, LGMD2H, TATIP
Gene Symbol
TRIM32
UniProt
Additional TRIM32 Products
Product Documents for Human TRIM32 Antibody
Product Specific Notices for Human TRIM32 Antibody
For research use only
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