Human TrkB Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3971U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 72509
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkB
Cys32-His430
Accession # Q16620
Cys32-His430
Accession # Q16620
Specificity
Detects human TrkB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TrkA, recombinant rat TrkA, recombinant mouse (rm) TrkB, rhTrkC, or rmTrkC is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human TrkB Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Human TrkB transfected C6 cells
Sample: Human TrkB transfected C6 cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TrkB
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
References
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
UniProt
Additional TrkB Products
Product Documents for Human TrkB Alexa Fluor® 350-conjugated Antibody
Product Specific Notices for Human TrkB Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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