Human TrkB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3971
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry, ICC-IF, Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 72509
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkB
Cys32-His430
Accession # Q16620
Cys32-His430
Accession # Q16620
Specificity
Detects human TrkB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TrkA, recombinant rat TrkA, recombinant mouse (rm) TrkB, rhTrkC, or rmTrkC is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TrkB Antibody
Detection of TrkB in Baf3 cells by Flow Cytometry.
Baf3 cells transfected with Human TrkB (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human TrkB Monoclonal Antibody (Catalog # MAB3971) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.Applications for Human TrkB Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Baf3 cells transfected with Human TrkB vs Irrelevant transfectant cells
Sample: Baf3 cells transfected with Human TrkB vs Irrelevant transfectant cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkB
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
References
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
UniProt
Additional TrkB Products
Product Documents for Human TrkB Antibody
Product Specific Notices for Human TrkB Antibody
For research use only
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