Human TSH beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB57941
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 512908
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TSH beta
Phe21-Val138
Accession # NP_000540
Phe21-Val138
Accession # NP_000540
Specificity
Detects TSH beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat TSH beta was observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TSH beta Antibody
Detection of Human TSH beta by Western Blot.
Western blot shows purified human pituitary TSH beta (10 ng/lane). PVDF Membrane was probed with 2 µg/mL of Human TSH beta Monoclonal Antibody (Catalog # MAB57941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSH beta at approximately 25 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
TSH beta in Human Pituitary.
TSH beta was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Thyroid Stimulating Hormone Subunit Beta Monoclonal Antibody (Catalog # MAB57941) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of thyrotrope cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human TSH beta Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pituitary subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human pituitary subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
2 µg/mL
Sample: Purified human pituitary TSH beta under non-reducing conditions only
Sample: Purified human pituitary TSH beta under non-reducing conditions only
Reviewed Applications
Read 1 review rated 3 using MAB57941 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSH beta
TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature human TSH beta shares 89% and 90% aa sequence identity with mouse and rat TSH beta, respectively.
Long Name
Thyroid-stimulating Hormone Subunit beta
Alternate Names
Thyrotropin beta, TSHB
Gene Symbol
TSHB
UniProt
Additional TSH beta Products
Product Documents for Human TSH beta Antibody
Product Specific Notices for Human TSH beta Antibody
For research use only
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