Human TTF-1/NKX2-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9458
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2054B
Product Summary for Human TTF-1/NKX2-1 Antibody
Immunogen
E. coli-derived recombinant human TTF-1/NKX2-1
Ala40-Pro111
Accession # P43699
Ala40-Pro111
Accession # P43699
Specificity
Detects human TTF-1/NKX2-1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human TTF-1/NKX2-1 Antibody
Detection of Human TTF-1/NKX2-1 by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TTF-1/NKX2-1 in A549 Human Cell Line.
TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TTF-1/NKX2-1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line
Sample: TT human medullary thyroid cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TTF-1/NKX2-1
Long Name
NK2 Homeobox 1
Alternate Names
BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1
Gene Symbol
NKX2-1
UniProt
Additional TTF-1/NKX2-1 Products
Product Documents for Human TTF-1/NKX2-1 Antibody
Product Specific Notices for Human TTF-1/NKX2-1 Antibody
For research use only
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