Human TTF-1/NKX2-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB94581
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2054E
Product Specifications
Immunogen
E. coli-derived recombinant human TTF-1/NKX2-1
Ala40-Pro111
Accession # P43699
Ala40-Pro111
Accession # P43699
Specificity
Detects human TTF-1/NKX2-1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human TTF-1/NKX2-1 Antibody
Detection of Human TTF-1/NKX2-1 by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TTF-1/NKX2-1 in A549 Human Cell Line.
TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TTF-1/NKX2-1 in Human Thyroid.
TTF-1/NKX2-1 was detected in immersion fixed paraffin-embedded sections of human thyroid using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TTF-1/NKX2-1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample:
Sample:
Immersion fixed A549 human lung carcinoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid
Sample: Immersion fixed paraffin-embedded sections of human thyroid
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line
Sample: TT human medullary thyroid cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB94581 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TTF-1/NKX2-1
Long Name
NK2 Homeobox 1
Alternate Names
BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1
Gene Symbol
NKX2-1
UniProt
Additional TTF-1/NKX2-1 Products
Product Documents for Human TTF-1/NKX2-1 Antibody
Product Specific Notices for Human TTF-1/NKX2-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...