Human Twist-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6230R
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-His202
Accession # Q15672
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Twist-1 Antibody
Detection of Human Twist‑1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Twist-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Twist‑1 in A549 Human Cell Line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti-Human Twist-1 Monoclonal Antibody (Catalog # MAB6230R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human Twist-1 Antibody
CyTOF-ready
Intracellular Staining by Flow Cytometry
Sample: A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB6230R in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Twist-1
Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Twist-1 Products
Product Documents for Human Twist-1 Antibody
Product Specific Notices for Human Twist-1 Antibody
For research use only