Detects human Tyro3/Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interferance with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, or rhMer is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Tyro3/Dtk Antibody

Detection of Dtk in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Tyro3/Dtk Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Tyro3/Dtk knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human Tyro3/Dtk Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Knockout Validated

Tyro3/Dtk is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in Tyro3/Dtk knockout K562 cell line.

Human Tyro3/Dtk Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human Tyro3/Dtk Biotinylated Antibody (Catalog # BAF859)
Standard: Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (Catalog # 859-DK)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Dtk (also called Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

Q06418

Additional Tyro3/Dtk Products

Product Documents for Human Tyro3/Dtk Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Tyro3/Dtk Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Tyro3/Dtk Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB859

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications