Human Ubiquitin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8595
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Bacteria
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1002A
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human Ubiquitin
Accession # P0CG47
Accession # P0CG47
Specificity
Detects human Ubiquitin in direct ELISAs
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Ubiquitin Antibody
Detection of Human Ubiquitin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Ubiquitin at various molecular weights (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Ubiquitin by Western Blot.
Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 5 ng/lane. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Di-Ubiquitin as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Ubiquitin Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG132
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG132
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB8595 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ubiquitin
Additional Ubiquitin Products
Product Documents for Human Ubiquitin Antibody
Product Specific Notices for Human Ubiquitin Antibody
For research use only
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