Human UCH-L3 Antibody

Name: Human UCH-L3 Antibody
Brand: R&D Systems
SKU: MAB6008

R&D Systems, part of Bio-Techne | Catalog # MAB6008

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 677710

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Product Summary for Human UCH-L3 Antibody

Immunogen

E. coli-derived recombinant human UCH-L3
Glu2-Ala230
Accession # P15374

Specificity

Detects human UCH-L3 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UCH-L1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human UCH-L3 Antibody

Detection of Human UCH‑L3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human UCH-L3 Monoclonal Antibody (Catalog # MAB6008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human UCH-L3 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human UCH-L3, see our available Western blot detection antibodies

Western Blot

3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HT‑29 human colon adenocarcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L3

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH‑L3 cleaves both monomeric ubiquitin (Ub) from Ub‑protein conjugates, and a GGLRQ peptide from the C‑terminus of the Ub‑like protein NEDD‑8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130 and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179‑230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full length human UCH-L3 shares 98% aa identity with mouse and rat UCH‑L3.

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Entrez Gene IDs

7347 (Human); 50933 (Mouse); 114094 (Rat); 100350137 (Rabbit)

Gene Symbol

UCHL3

UniProt

P15374 (Human)

Additional UCH-L3 Products

Product Documents for Human UCH-L3 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human UCH-L3 Antibody

For research use only

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