Human Urocortin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7447
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 749606
Product Specifications
Immunogen
E. coli-derived recombinant human Urocortin
Arg26-Lys124 (predicted)
Accession # P55089
Arg26-Lys124 (predicted)
Accession # P55089
Specificity
Detects human Urocortin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Urocortin Antibody
Detection of Human Urocortin by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Urocortin at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Urocortin in Human Brain.
Urocortin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Urocortin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Western Blot
0.5 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line
Sample: SH‑SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Urocortin
Alternate Names
UCN, UI, UROC
Gene Symbol
UCN
UniProt
Additional Urocortin Products
Product Documents for Human Urocortin Antibody
Product Specific Notices for Human Urocortin Antibody
For research use only
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