Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of UVRAG in Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human UVRAG Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UVRAG

UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.

Long Name

UV Radiation Resistance Associated

Alternate Names

DHTX, VPS38

Entrez Gene IDs

7405 (Human); 78610 (Mouse); 308846 (Rat)

Gene Symbol

UVRAG

UniProt

Q9P2Y5

Additional UVRAG Products

Product Documents for Human UVRAG Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human UVRAG Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human UVRAG Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9180

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all UVRAG Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human UVRAG Antibody

Detection of UVRAG in Raji Human Cell Line by Flow Cytometry.