Human VEGFR1/Flt-1 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB321
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Cell Culture, ELISA Development, Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 49560
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VEGFR1/Flt-1 Antibody
Detection of VEGFR1/Flt‑1 in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human VEGFR1/Flt-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
1 µg/mL
Sample: Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only
Sample: Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL)
under non-reducing conditions only
Reviewed Applications
Read 2 reviews rated 5 using MAB321 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR1/Flt-1
Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
UniProt
Additional VEGFR1/Flt-1 Products
Product Documents for Human VEGFR1/Flt-1 Antibody
Product Specific Notices for Human VEGFR1/Flt-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...