Human VEGFR2/KDR/Flk-1 PE-conjugated Antibody

Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 PE-conjugated Mono-clonal Antibody (Catalog # FAB357P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human VEGFR2/KDR/Flk-1 by Flow Cytometry

Circulating EPC number in healthy controls and diabetes. a and b: Circulating EPC numbers were determined by flow cytometry for the co-expression of CD34 and VEGFR2 (b). Peripheral blood MNCs incubated with IgG isotype control (a) serve as a negative control to determine the intrinsic fluorescent intensity of the peripheral blood MNCs and to define positive area R2. c: Absolute number of circulating EPCs in healthy controls and diabetes as determined by the co-expression of CD34 and VEGFR2. d: Absolute number of circulating EPCs in healthy controls, diabetes with good and poor glycemic control as determined by the co-expression of CD34 and VEGFR2. e: Correlation between the circulating EPC numbers and FBS, f: Correlation between the circulating EPC numbers and HbA1C, g: Absolute number of circulating CD34+/VEGFR2- cells in healthy controls and diabetes. Data are presented as means ± SEM. Image collected and cropped by CiteAb from the following publication (https://bmcendocrdisord.biomedcentral.com/articles/10.1186/1472-6823-10-5), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human VEGFR2/KDR/Flk-1 by Flow Cytometry

Phenotypic characterization of cultured PACs. A) PACs were characterized for surface marker expression by flow cytometry. In the side scatted (SSC) versus forward scatter (FSC) morphologic plot, lymphocytic cells (LYMPHs) and monocyte-macrophages (MONOs) were identified and gated separately. Histograms reporting the expression of relevant leukocyte (CD45, CD14, CD68) and endothelial markers (CD31, KDR, CD34) are shown, together with mean percent expression from 3 replicates. The red line indicates negative control, while the blue line indicates the stained condition. B) Cells in the PACs culture were stained with the endothelial markers acLDL and Ulex Lectin. The fraction of cells that were positive for both markers were compared in cultures obtained from T2D or healthy control cells. *p < 0.05 T2D vs Ctrl. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24886621), licensed under a CC-BY license. Not internally tested by R&D Systems.