Human VHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB16541
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 237018
Product Specifications
Immunogen
E. coli-derived recombinant human VHR
Ser2-Pro185
Accession # P51452
Ser2-Pro185
Accession # P51452
Specificity
Detects human VHR in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human VHR Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung and liver
Sample: Immersion fixed paraffin-embedded sections of human lung and liver
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VHR
Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).
References
- Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
- Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
- Alonso, A. et al. (2003) Nat. Immunol. 4:44.
- Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
- Yuvaniyama, J. et al. (1996) Science 272:1328.
- Ueda, K. et al. (2002) FEBS Lett. 525:48.
Long Name
Vaccinia Virus VH1-related Phosphatase
Alternate Names
DUSP3
Entrez Gene IDs
1845 (Human)
Gene Symbol
DUSP3
UniProt
Additional VHR Products
Product Documents for Human VHR Antibody
Product Specific Notices for Human VHR Antibody
For research use only
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