Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human VMAT2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VMAT2 at approximately 75-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of VMAT2 in HEK293 Human Cell Line Transfected with Human VMAT2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human VMAT2 and eGFP was stained with either (A) Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

VMAT2 in Human Brain.

VMAT2 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human VMAT2 Monoclonal Antibody (Catalog # MAB8327) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human VMAT2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human VMAT2 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line either mock transfected or transfected with human VMAT2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VMAT2

The Vesicular Monoamine Transporter 2 (VMAT2), also known as VAT2 and SCL18A, is a 55-75 kDa member of the vesicular transporter family, a major facilitator superfamily. VMAT2 is a 12 transmembrane (TM) glycoprotein that is found in the membrane of brain neurosecretory vesicles. It transports monoamines (dopamine, serotonin, and particularly histamine) from the cytosol into secretion vesicles by exchanging two H⁺ ions for one molecule of amine. Human VMAT2 is 514 amino acids (aa) in length. It contains two cytoplasmic domains, a 20 aa and a 52 aa N- and C-terminal respectively, plus an extended 88 aa luminal loop between aa 42-129. There is one luminal, intrachain disulfide bond that contributes to amine transport (C126-C333). In addition, residues in TM domains 5-8 (aa 220-352) and 9-12 (aa 358-462) also contribute to high affinity ligand interaction. VMAT2 is constitutively phosphorylated by CKII on S511 and S513. Within the cytoplasmic C-terminus, human VMAT2 is 94% aa identical to rat VMAT2.

Long Name

Vesicular Monoamine Transporter 2/Solute Carrier Family 18, Member 2

Alternate Names

MNAT, SLC18A2, SVAT, SVMT, VAT2

Entrez Gene IDs

6571 (Human); 214084 (Mouse); 25549 (Rat)

Gene Symbol

SLC18A2

UniProt

Q05940

Additional VMAT2 Products

Product Documents for Human VMAT2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human VMAT2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VMAT2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8327

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all VMAT2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VMAT2 Antibody

Detection of Human VMAT2 by Western Blot.