HEK293 human embryonic kidney cell line transfected with (A) human VSIG2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG2 Monoclonal Antibody (Catalog # MAB51771) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human VSIG2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human VSIG2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as cortical thymocyte-like protein (CT-like protein), is a member of the immunoglobulin superfamily (Igsf) that was originally described in Xenopus and was referred to as cortical thymocyte marker in Xenopus, or CTX. The clone isolated from the human genomic library was called CTH (1). Mature human VSIG2 is a single-pass type I transmembrane protein with a 220 amino acid (aa) extracellular domain (ECD) that contains one C2-type and one V-type immunoglobulin-like domains, and a 63 aa cytoplasmic region linked to the ECD by a short 21 aa transmembrane domain. Human VSIG2 ECD shares 85% and 86% aa sequence identity with mouse and rat VSIG2, respectively. VSIG2 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG2 inhibits T cell activation, including IL17 and interferon gamma production.

References

Chrétien, I. et al. (1998) Eur. J. Immunol. 28:4094.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Alternate Names

CTH, CTXL

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Gene Symbol

VSIG2

UniProt

Q961Q7-1

Additional VSIG2 Products

Product Documents for Human VSIG2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human VSIG2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VSIG2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB51771

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all VSIG2 Antibodies »

Product Summary for Human VSIG2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VSIG2 Antibody

Detection of VSIG2 in HEK293 Human Cell Line Transfected with Human VSIG2 and eGFP by Flow Cytometry.