Human VSIG3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11226
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2637B
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Gly129-Cys138
Accession # Q5DX21
Gly129-Cys138
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human VSIG3 Antibody
Detection of Human VSIG3 by Simple WesternTM.
Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for VSIG3 at approximately 57 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human VSIG3 by Western Blot.
Western blot shows lysates of IMR-32 human neuroblastoma cell line, SH-SY5Y human neuroblastoma cell line and Mouse cerebellum. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human VSIG3 Antibody
Application
Recommended Usage
Simple Western
0.2 µg/mL
Sample: IMR‑32 human neuroblastoma cell line
Sample: IMR‑32 human neuroblastoma cell line
Western Blot
2 µg/mL
Sample: IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum
Sample: IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG3
VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Long Name
V-Set and Immunoglobulin Domain Containing 3
Alternate Names
BTIGSF, CXADRL1, IGSF11
Gene Symbol
IGSF11
UniProt
Additional VSIG3 Products
Product Documents for Human VSIG3 Antibody
Product Specific Notices for Human VSIG3 Antibody
For research use only
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