Human WTX Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7374
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 729419
Product Summary for Human WTX Antibody
Immunogen
E. coli-derived recombinant human WTX
Met421-Leu573
Accession # Q5JTC6
Met421-Leu573
Accession # Q5JTC6
Specificity
Detects human WTX in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human WTX Antibody
Detection of Human WTX by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
WTX in BG01V Human Embryonic Stem Cells.
WTX was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human WTX Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WTX
Long Name
Wilms Tumor on the X Chromosome
Alternate Names
AMER1, FAM123B, OSCS
Gene Symbol
AMER1
UniProt
Additional WTX Products
Product Documents for Human WTX Antibody
Product Specific Notices for Human WTX Antibody
For research use only
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