Human XIAP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8221
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Neutralization
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 117318
Product Specifications
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Met1-Ser497
Accession # P98170
Specificity
Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human XIAP Antibody
XIAP in Human Brain.
XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization of XIAP Activity by Human XIAP Antibody.
Recombinant Human Caspase-7 (0.012 µg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) that has been preincubated with increasing concentrations of Mouse Anti-Human XIAP Antibody (Catalog # MAB8221) The ND50 is typically 10 µg/mL.Applications for Human XIAP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Reviewed Applications
Read 2 reviews rated 3 using MAB8221 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 6 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XIAP
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Human XIAP Antibody
Product Specific Notices for Human XIAP Antibody
For research use only
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