ICAM-1/CD54 Antibody (MEM-111) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-318B

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Product Datasheet / COA / SDS

Clone MEM-111 was used by HLDA to establish CD designation.

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB500-318AF350

Alexa Fluor 405

NB500-318AF405

Alexa Fluor 488

NB500-318AF488

Alexa Fluor 532

NB500-318AF532

Alexa Fluor 594

NB500-318AF594

Alexa Fluor 647

NB500-318AF647

Alexa Fluor 700

NB500-318AF700

Alexa Fluor 750

NB500-318AF750

Allophycocyanin

NB500-318APC

Allophycocyanin/Cy7

NB500-318APCCY7

CoraFluor 1

NB500-318CL1

DyLight 350

NB500-318UV

DyLight 405

NB500-318V

DyLight 488

NB500-318G

DyLight 550

NB500-318R

DyLight 594

NB500-318DL594

DyLight 650

NB500-318C

DyLight 680

NB500-318FR

DyLight 755

NB500-318IR

FITC

NB500-318F

HRP

NB500-318H

Janelia Fluor 525

NB500-318JF525

Janelia Fluor 549

NB500-318JF549

Janelia Fluor 585

NB500-318JF585

Janelia Fluor 635

NB500-318JF635

Janelia Fluor 646

NB500-318JF646

Janelia Fluor 669

NB500-318JF669

mFluor Violet 450 SE

NB500-318MFV450

mFluor Violet 500 SE

NB500-318MFV500

mFluor Violet 610 SE

NB500-318MFV610

PE

NB500-318PE

PE/Atto594

NB500-318PEATT594

PE/Cy5.5

NB500-318PECY55

PE/Cy7

NB500-318PECY7

PerCP

NB500-318PCP

Forumulation

Catalog #

BSA Free

NB500-318

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG_2A Clone # MEM-111

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ICAM-1/CD54 Antibodies »

Product Summary for ICAM-1/CD54 Antibody (MEM-111) [Biotin]

Immunogen

Raji human Burkitt's lymphoma cell line (NM_000201.2).

Specificity

The antibody MEM-111 reacts with an extracellular epitope CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for ICAM-1/CD54 Antibody (MEM-111) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (MEM-111) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for ICAM-1/CD54 Antibody (MEM-111) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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