ICAM-1/CD54 Antibody (MEM-111) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-318PE
Clone MEM-111 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Bovine
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # MEM-111
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ICAM-1/CD54 Antibody (MEM-111) [PE]
Immunogen
Raji human Burkitt's lymphoma cell line (NM_000201.2).
Specificity
The antibody MEM-111 reacts with an extracellular epitope CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for ICAM-1/CD54 Antibody (MEM-111) [PE]
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [PE] [NB500-318PE] - A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.
Applications for ICAM-1/CD54 Antibody (MEM-111) [PE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for ICAM-1/CD54 Antibody (MEM-111) [PE]
Product Specific Notices for ICAM-1/CD54 Antibody (MEM-111) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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