IgG Antibody (RM104) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62005B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Western Blot

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM104

Concentration

1 mg/ml

View all IgG Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG Antibody (RM104) [Biotin]

Western Blot: IgG Antibody (RM104) [Biotin] [NBP2-62005B] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20 ng/lane), using 0.2 ug/mL of clone #104 (NBP2-62005B). This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA: IgG Antibody (RM104) [Biotin] [NBP2-62005B] - ELISA of mouse immunoglobulins shows NBP2-62005B reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with different immunoglobulins. NBP2-62005B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG Antibody (RM104) [Biotin] [NBP2-62005B] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62005B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG Antibody (RM104) [Biotin]

Application
Recommended Usage

ELISA

0.005 ug/ml-0.2 ug/ml

Western Blot

0.1 ug/ml - 0.5 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for IgG Antibody (RM104) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgG Antibody (RM104) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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