IgG Antibody (RM104) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25949
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM104
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for IgG Antibody (RM104) - Azide and BSA Free
Immunogen
Mouse IgG
Specificity
This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG Antibody (RM104) - Azide and BSA Free
Western Blot: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] -
Western Blot: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20ng/lane), using Clone RM104. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.
ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] -
ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] -
ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP3-25949] - ELISA of mouse immunoglobulins shows RM104 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG Antibody (RM104) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.005 ug/ml-0.2 ug/ml
Western Blot
0.1 ug/ml-0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for IgG Antibody (RM104) - Azide and BSA Free
Product Specific Notices for IgG Antibody (RM104) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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