IgG3 Antibody (RM218) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62010B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM218
Concentration
1 mg/ml
Product Summary for IgG3 Antibody (RM218) [Biotin]
Immunogen
Mouse IgG3
Specificity
This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG3 Antibody (RM218) [Biotin]
ELISA: IgG3 Antibody (RM218) [Biotin] [NBP2-62010B] - ELISA of mouse immunoglobulins shows NBP2-62010B reacts to both mouse IgG3k and IgG3 lambda. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins. NBP2-62010B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG3 Antibody (RM218) [Biotin] [NBP2-62010B] - A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of NBP2-62010B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for IgG3 Antibody (RM218) [Biotin]
Application
Recommended Usage
ELISA
0.005 ug/ml-0.2 ug/ml
Western Blot
0.1 ug/ml - 0.5 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG3
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene Symbol
IGHG3
Additional IgG3 Products
Product Documents for IgG3 Antibody (RM218) [Biotin]
Product Specific Notices for IgG3 Antibody (RM218) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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