IgG3 Antibody (RM218) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25954
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM218
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for IgG3 Antibody (RM218) - Azide and BSA Free
Immunogen
Mouse IgG3
Specificity
This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG3 Antibody (RM218) - Azide and BSA Free
ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] -
ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] - A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] -
ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP3-25954] - ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3 kappa and IgG3 lambda; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG3 Antibody (RM218) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.005 ug/ml-0.2 ug/ml
Western Blot
0.1 ug/ml - 0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG3
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene Symbol
IGHG3
Additional IgG3 Products
Product Documents for IgG3 Antibody (RM218) - Azide and BSA Free
Product Specific Notices for IgG3 Antibody (RM218) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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