IgM Antibody (RM121) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62022B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM121
Concentration
1 mg/ml
Product Summary for IgM Antibody (RM121) [Biotin]
Immunogen
Human IgM
Specificity
This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgM Antibody (RM121) [Biotin]
ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - ELISA of human immunoglobulins shows NBP2-62022B reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with different immunoglobulins. NBP2-62022B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - Sandwich ELISA using NBP2-62022B as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - Sandwich ELISA using NBP2-62022B as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (kappa + lamda) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Applications for IgM Antibody (RM121) [Biotin]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for IgM Antibody (RM121) [Biotin]
Product Specific Notices for IgM Antibody (RM121) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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