IgM Antibody (RM121) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62022B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM121

Concentration

1 mg/ml

View all IgM Antibodies »

Product Specifications

Immunogen

Human IgM

Specificity

This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgM Antibody (RM121) [Biotin]

ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - ELISA of human immunoglobulins shows NBP2-62022B reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with different immunoglobulins. NBP2-62022B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - Sandwich ELISA using NBP2-62022B as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA: IgM Antibody (RM121) [Biotin] [NBP2-62022B] - Sandwich ELISA using NBP2-62022B as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (kappa + lamda) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Applications for IgM Antibody (RM121) [Biotin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (RM121) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgM Antibody (RM121) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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