IgM Antibody (RM121) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25968
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM121
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for IgM Antibody (RM121) - Azide and BSA Free
Immunogen
Human IgM
Specificity
This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgM Antibody (RM121) - Azide and BSA Free
ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] -
ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] - A titer ELISA using RM121. The plate was coated with different amounts of human IgM. A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] -
ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] - ELISA of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Sandwich ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] -
Sandwich ELISA: IgM Antibody (RM121) - Azide and BSA Free [NBP3-25968] - Sandwich ELISA using RM121 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Applications for IgM Antibody (RM121) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5 - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for IgM Antibody (RM121) - Azide and BSA Free
Product Specific Notices for IgM Antibody (RM121) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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