Ikaros/IKZF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51990

Novus Biologicals, part of Bio-Techne
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NBP1-51990
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ikaros Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1.

Epitope

C-DTPYDSSASYEKEN

Specificity

This antibody is expected to recognise all isoforms NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ikaros/IKZF1 Antibody

Ikaros/IKZF1 Antibody

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] -

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Ikaros/IKZF1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:1000
Application Notes
WB: Preliminary testing showed a band at approx. 60kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1ug/ml antibody staining (calculated MW of 57.5kDa according to NP_006051.1). Primary incubation 1 hour at room temperature. IHC-P: Human tonsil shows textured staining in some nuclei of the lymphoid tissue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ikaros

IKZF1 is a transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).

Long Name

DNA-binding protein Ikaros

Alternate Names

IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1

Entrez Gene IDs

10320 (Human)

Gene Symbol

IKZF1

UniProt

NP_006051.1 (Human)

Additional Ikaros Products

Product Documents for Ikaros/IKZF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ikaros/IKZF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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