Ikaros/IKZF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51990

Novus Biologicals, part of Bio-Techne
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NBP1-51990
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ikaros Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1.

Epitope

C-DTPYDSSASYEKEN

Specificity

This antibody is expected to recognise all isoforms NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ikaros/IKZF1 Antibody

Ikaros/IKZF1 Antibody

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] -

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Ikaros/IKZF1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:1000
Application Notes
WB: Preliminary testing showed a band at approx. 60kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1ug/ml antibody staining (calculated MW of 57.5kDa according to NP_006051.1). Primary incubation 1 hour at room temperature. IHC-P: Human tonsil shows textured staining in some nuclei of the lymphoid tissue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ikaros

IKZF1 is a transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).

Long Name

DNA-binding protein Ikaros

Alternate Names

IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1

Entrez Gene IDs

10320 (Human)

Gene Symbol

IKZF1

UniProt

NP_006051.1 (Human)

Additional Ikaros Products

Product Documents for Ikaros/IKZF1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ikaros/IKZF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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